Involvement of formyl peptide receptors in the stimulatory effect of crotoxin on macrophages co-cultivated with tumour cells

Crotoxin (CTX) is the main neurotoxic component of Crotalus durissus terrificus snake venom. It inhibits tumour growth and modulates the function of macrophages, which are essential cells in the tumour microenvironment. the present study investigated the effect of CTX on the secretory activity of monocultured macrophages and macrophages co-cultivated with LLC-WRC 256 cells. the effect of the macrophage secretory activities on tumour cell proliferation was also evaluated. Macrophages pre-treated with CTX (0.3 mu g/mL) for 2 h were co-cultivated with LLC-WRC 256 cells, and the secretory activity of the macrophages was determined after 12, 24 and 48 h. the co-cultivation of CTX-treated macrophages with the tumour cells caused a 20% reduction in tumour cell proliferation. the production of both H2O2 and NO was increased by 41% and 29% after 24 or 48 h of co-cultivation, respectively, compared to the values for the co-cultures of macrophages of control. the level of secreted IL-1 beta increased by 3.7- and 3.2-fold after 12 h and 24 h of co-cultivation, respectively. Moreover, an increased level of LXA(4) (25%) was observed after 24 h of co-cultivation, and a 2.3- and 2.1-fold increased level of 15-epi-LXA(4) was observed after 24 h and 48 h, respectively. Boc-2, a selective antagonist of formyl peptide receptors, blocked both the stimulatory effect of CTX on the macrophage secretory activity and the inhibitory effect of these cells on tumour cell proliferation. Taken together, these results indicate that CTX enhanced the secretory activity of macrophages, which may contribute to the antitumour activity of these cells, and that activation of formyl peptide receptors appears to play a major role in this effect. (C) 2013 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)PAPInstituto Nacional de Ciencia e Tecnologia em ToxinasButantan Inst, Lab Pathophysiol, BR-05503900 São Paulo, BrazilUniversidade Federal de São Paulo, Lab Inflammat & Vasc Pharmacol, BR-09913030 Diadema, SP, BrazilUniv São Paulo, Fac Med, BR-01246000 São Paulo, BrazilUniv São Paulo, Inst Biomed Sci, Dept Anat, BR-05508900 São Paulo, BrazilUniv São Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 São Paulo, BrazilButantan Inst, Special Lab Pain & Signaling, BR-05503900 São Paulo, BrazilUniversidade Federal de São Paulo, Lab Inflammat & Vasc Pharmacol, BR-09913030 Diadema, SP, BrazilFAPESP: 09/52330-9Instituto Nacional de Ciencia e Tecnologia em Toxinas: INCTTOX 2008/57898-0Web of Scienc

A perturbative approach to calculating the Casimir force in fluctuating scalar and vector fields

Based on a perturbative approach, a series expansion in susceptibility function of the medium is obtained for the Casimir force between arbitrary shaped objects immersed in a scalar or vector fluctuating field in arbitrary dimensions. Finite-temperature corrections are derived and the results are compared in first order with weak coupling regime in scattering method. The generalization to a massive vector field is also investigated.Comment: 20 pages, two figure

Science verification of the new FlashCam-based camera in the 28m telescope of H.E.S.S

In October 2019 the central 28m telescope of the H.E.S.S. experiment has beenupgraded with a new camera. The camera is based on the FlashCam design whichhas been developed in view of a possible future implementation in themedium-sized telescopes of the Cherenkov Telescope Array (CTA). We report hereon the results of the science verification program that has been performedafter commissioning of the new camera, to show that the camera and softwarepipelines are working up to expectations.<br

Perception of Perspective Distortions in Image-Based Rendering

International audienceImage-based rendering (IBR) creates realistic images by enriching simple geometries with photographs, e.g., mapping the photograph of a building façade onto a plane. However, as soon as the viewer moves away from the correct viewpoint, the image in the retina becomes distorted, sometimes leading to gross misperceptions of the original geometry. Two hypotheses from vision science state how viewers perceive such image distortions, one claiming that they can compensate for them (and therefore perceive scene geometry reasonably correctly), and one claiming that they cannot compensate (and therefore can perceive rather significant distortions). We modified the latter hypothesis so that it extends to street-level IBR. We then conducted a rigorous experiment that measured the magnitude of perceptual distortions that occur with IBR for façade viewing. We also conducted a rating experiment that assessed the acceptability of the distortions. The results of the two experiments were consistent with one another. They showed that viewers' percepts are indeed distorted, but not as severely as predicted by the modified vision science hypothesis. From our experimental results, we develop a predictive model of distortion for street-level IBR, which we use to provide guidelines for acceptability of virtual views and for capture camera density. We perform a confirmatory study to validate our predictions, and illustrate their use with an application that guides users in IBR navigation to stay in regions where virtual views yield acceptable perceptual distortions